In spite of the recent developments in structural biology, membrane proteins continue to pose tremendous challenges to most biophysical techniques. A major area of research in my group is focused on the development of NMR and other biophysical/biochemical approaches to study the dynamic structures of membrane-bound proteins such as cytochrome P450, cytochrome b5 and cytochrome P450-reductase.[1] Strategies to study the structure and dynamics of these challenging systems and also on the electron transfer mechanism that enables the enzymatic function of P450 will be presented in my talk. A variety of DNP-MAS and oriented Solid-state NMR approaches to measure the transmembrane-transmembrane interactions will be highlighted. Structural interactions of cytochrome-P450 with its REDOX partners, lipid membrane, and substrates investigated using novel peptide-based and polymer-based lipid nanodiscs/macrodiscs will also be presented.[2]

Role of membrane on the function of cytchrome-P450

254th National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.