Reversible addition fragmentation chain transfer (RAFT) polymerization and atom transfer radical polymerization (ATRP) were employed to synthesize biomimetic polymers. These polymers were rationally designed to stabilize proteins both by increasing pharmacokinetics and stability to storage conditions such as fluctuations to temperature and solution agitation. The polymers can be made with or without end groups by using functionalized chain transfer agents or initiators. Thus, they can be added or conjugated to proteins. Studies of protein stabilization to aggregation and denaturation in the presence of the polymers were undertaken. Investigation of protein activity in vitro and in vivo in the presence of polymer attached or conjugated were completed. The protein-polymer conjugates in particular were subjected to in vivo pharmacokinetic tests to quantify blood plasma levels over time. The synthesis of the polymers and conjugates, and the results of the stability and biological investigations will be presented.

CRP polymers that stabilize therapeutic proteins in vivo and during storage

254th National Meeting (2017)

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