Biohybrid polymers are copolymers synthesized by covalent linking of natural (e.g. proteins) and synthetic polymers. Enzymes offer a perfect scaffold for the generation of polymeric biohybrids capable of performing a specific functionality. Polymer based protein engineering (PBPE) allows the development of versatile conjugates beyond the traditional pegylation. Besides, PBPE techniques are capable of conferring to the enzymatic biohybrids new properties that can trigger a change in their physical properties, have a superior performance, proteolytic stability, and improved activity in aqueous/organic solvents. We have performed the modification of Lipase (<i>Thermomyces Lanuginosus</i>), which is of significant interest for the biofuel, food, detergent, pharmaceutical, leather, textile, cosmetic, and paper industries. The challenges and advantages of the structural modification of the surface of lipase with thermoresponsive polyacrylamides (DMAPAAm, SBAAm, and NIPAM) will be presented. They are expected to improve the recovery of the catalytic entity and yield stability under harsh conditions. The successful polymerization conducted via aqueous AGET-ATRP using a “grafting from” approach and studies related to catalytic behavior will be addressed.

Synthesis and design of smart protein-polymer biohybrids via ATRP in water

254th National Meeting (2017)

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