A non-enzymatic glucose biosensor was developed by using two dimensional assemblies of gold (III) nanoparticles (HAuCl4) generated by in situ reduction of [HAuCl4]- ions attached to polyethyleneimine silane functionalized silicon and indium tin oxide (ITO) coated glass surfaces. Cyclic voltammetry (CV) and impedance spectroscopy (IS) were used to detect glucose (C6H12O6). The range detected for glucose was observed from 0.01mM to 0.1nM using both cyclic voltammetry and impedance spectroscopy in the presence of sodium hydroxide buffer. Cyclic voltammetry resulted in an increase in current (A) as concentration increased above the sodium hydroxide buffer alone. Impedance spectroscopy showed an increase in resistance (ohm) and a decrease in capacitance (F) as concentration increases. The low detection limit was observed at 0.1nM glucose for both methods used. The advantage this application possesses includes more accurate glucose detection without the use of enzymes. The gold nanoparticle coated indium tin oxide surfaces allows for highly sensitive and more accurate detection of glucose which can lead to further developments in more accurate ways to detect blood glucose levels in diabetes patients.

Two dimensional assemblies of gold nanoparticle as non-enzymatic glucose biosensor

254th National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.