Perilla frutescens leaves is valuable as a medicinal plant as well as a natural medicine and functional food. Perilla leaves are an important source of essential oils and had antidepressive, anxiolytic, chemopreventive and strong antitumor-promoting activities. Four different isolation techniques, specifically ultrasound-assisted supercritical carbon dioxide (USC-CO2) extraction, heat-reflux extraction (HRE), conventional supercritical carbon dioxide (SC-CO2) extraction and hydrodistillation (HD) were employed to obtain essential oils from purple-leafed of Perilla frutescens. The essential oils were further analyzed using gas chromatography (GC) with flame ionization detection and gas chromatography with mass spectrometry (GC-MS). The maximum yield of oil was extracted via USC-CO2 extraction (1.92%, weight of the extracted oil/weight of feeding material) while utilizing less severe operating parameters, such as temperature (48 °C), pressure (25 MPa), organic solvent (0 mL) and the time consumed (85 min) by the process. The results revealed that the dominant component was perillaldehyde (&gt;25%) in total average content.

Development of a green alternative procedure for simultaneous separation and quantification of phytochemicals

254th National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.