The advent of electrospray ionization heralded a new era in mass spectrometry in the early 90’s. Liquid chromatography-tandem mass spectrometry transformed the sciences of metabolism, pharmacology and toxicology. The adoption of high resolution accurate mass (HRAM) instrumentation over the last decade has resulted in another surge in analytical capabilities allowing for continued advances in our understanding of biological systems and disease states.<br/>Orbitrap technology has provided researchers with instrumentation that allows extreme resolution and mass accuracy in instrumentation that is robust, highly flexible and easy to use. The application of HRAM in combination with tandem mass spectrometry and sophisticated data dependent acquisition techniques supports a diverse range of applications that allow researchers to probe biological systems with great sensitivity, specificity and speed. Quantitative proteomic techniques are used to identify processes that are up and down regulated in an unbiased manner to determine protein biomarkers. Metabolomics studies can be used to monitor disease states and identify on target and off target toxicities. Highly sensitive biomarker assays may be developed to identify disease and monitor the efficacy of drugs to treat disease states. HRAM techniques are used at all stages of drug discovery and development to monitor target engagement, optimize drug candidates, determine PK/PD relationships and identify toxicities.<br/>Recent examples of how the latest HRAM technologies are applied to drug and biomarker quantitation, structural elucidation, ADME studies, toxicology and translational medicine will be demonstrated.<br/>

Advances in mass spectrometry techniques for metabolism, pharmacology and toxicology

254th National Meeting (2017)

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