Hydrophilic interaction liquid chromatography (HILIC) has become popular for separation of polar compounds in the past two decades. The main reasons are its excellent compatibility with mass spectrometry (MS) and its straightforward nature compared to other chromatography techniques. There is a clear trend toward a HILIC-MS combination becoming a standard for the analysis of large quantities of metabolites in metabolomics studies in the future. Due to the complexity of such applications, there are urgent demands for new HILIC columns with durable stability, high reproducibility, and unique selectivity.<br/>We recently developed three novel charge-modulated amide HILIC materials based on spherical silica/polymer particles and innovative surface bonding technologies. These materials are covalently bonded with multiple hydrophilic functional groups: neutral, positively charged, and negatively charged, with different charge states and complementary selectivity. It has been suggested that the separation mechanism with these columns is based on a combination of hydrophilic interaction, hydrogen bonding, and weak electrostatic interactions. In this presentation, I will describe the separation selectivity and the factors for optimizing chromatographic separation. I will also discuss the advantages of using tailor-made selectivity to address difficult applications in metabolomics, lipidomics, pharma, and food and beverage analysis.<br/>

Advances in HILIC selectivity with tailor-made columns for HPLC and UHPLC

254th National Meeting (2017)

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