Three-dimensional culture models are more representative of in vivo cellular phenotypes and responses than the monolayer cultures traditionally used in research laboratories. We have developed a culture system in which we can engineer tumor-like environments with specific oxygen and nutrient profiles. The experimental control afforded by these 3D cultures, which are modular in format and able to incorporate many cell types and extracellular matrices, allow us to ask specific questions about oxygen (redox) biology. We are particularly interested in quantifying the relationship between oxygen tension, cellular metabolism, and susceptibility to chemotherapeutics. In this talk, I will highlight our recent advances in constructing breast and colon tumor models for studying drug metabolism. In particular, how sub-populations of cells in low oxygen tensions evade chemotherapeutics.

Paper-Based tumor models: Quantifying the role of oxygen in drug metabolism

254th National Meeting (2017)

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