Nanomaterials are gaining interest and advancing towards a wide range of biomedical applications. Noble metal nanoparticles (NPs) are used in a variety of biosensing applications due to their size dependent optical properties.  Surface plasmon excitation of NPs principally underpins optical detection methods.  We and others have shown that chemically modified spherical gold NPs can be used as extrinsic labels for surface enhanced Raman scattering (SERS) detection of surface based assays.  Thus far, all assays have used chemically synthesized NPs and routes to modify these NPs are well established.  While methods to chemically synthesize metallic NPs are been established, researchers are developing alternative approaches to produce NPs using biological organisms.  In this presentation, we will describe our efforts to incorporate biogenic spherical silver metal NPs (bioAgNPs), produced by the fungus <i>Fusarium oxysporum</i>, as plasmonic labels for enhanced spectroscopy applications.  Biogenic silver NPs are stabilized with a surface corona that is unique relative to their chemically prepared counterparts.  This layer provides enhanced colloidal stability of bioAgNPs even under conditions of moderate ionic strength.  We will present our results that characterize this layer as a mixture peptides and carbohydrates.  Chemical properties of the corona presents interesting opportunities for surface modification of bioAgNPs.  Potential advantages of a native biomolecular layer include simpler or milder modification strategies compared to chemically prepared NPs.  We have demonstrated a variety of Raman reporter molecules can be incorporated into the layer and antibodies can be linked to the layer.  These two modifications enable use of bioAgNPs in sandwich immunoassays with SERS detection.  Our long-term goal is to apply these labels into a predictive diagnostic assay for prostate cancer treatment.  Our preliminary results for the measurement of the protein fragment, AR-V7, a biomarker for prostate cancer therapy effectiveness will be presented.

Biogenic silver nanoparticles for surface enhanced Raman scattering based biosensing

254th National Meeting (2017)

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