This lecture will focus on data generated with an ion source that facilitates simultaneous generation of positively charged sample ions by electrospray ionization and negatively charged reagent ions for both electron transfer dissociation (ETD) and ion-ion proton transfer (IIPT) reactions on Orbitrap mass spectrometers. Implementation of multiple C-trap fills for enhanced sensitivity will be discussed and both parallel peak parking, and ion ejection strategies to facilitate protein separation and enhanced sequence coverage of intact proteins will be described. Use of IIPT/ETD facilitates near complete sequence coverage on many intact proteins, including antibodies, and is ideally suited for locating multiple posttranslational modifications on the same protein molecule. Also discussed is the identification of posttranslationally modified, Class I MHC peptides that trigger central memory T-cells to kill cancer cells.<br/>

Instrumentation and methods for the identification and sequence analysis of (1) intact proteins on a chromatographic time-scale and (2) characterization tumor specific phosphopeptides for immunotherapy of cancer

254th National Meeting (2017)

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