G protein-coupled receptors (GPCRs) are known to be involved in the GPCR signal transduction system and regulate many essential physiological processes in organisms. The function of GPCR-intracellular signaling in the development of insecticide resistance in the mosquitoes Culex quinquefasciatus was investigated. Our RNA-interference (RNAi)-mediated functional studies revealed that knockdown of GPCRs and downstream effectors of G protein alpha-subunit (Gαs), adenylyl cyclase (AC) and protein kinase (PKA) genes in the GPCR signaling pathway led to significant decreases in both the expression of resistance P450 genes and mosquito resistance to permethrin. The role of GPCRs was further explored using transgenic lines of Drosophila melanogaster, in which the tolerance of Drosophila to permethrin and the expression of Drosophila resistance P450 genes were both increased. These results revealed the importance of the GPCR regulatory signaling pathway in governing P450 gene expression and P450-mediated insecticide resistance in mosquitoes.

GPCR regulatory signaling pathway: The mechnisms underlying insecticide resistance in mosquitoes

254th National Meeting (2017)

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