Reactive carbonyls are widespread species in living organisms with highly reactive carbonyl groups, and mainly known for their damaging effects on proteins, nucleic acids, and lipids. Accumulation of reactive carbonyl species (RCS) are closely linked to the developments of pathological disorders and diseases, such as diabetes, diabetic complications, and aging related diseases. To accurately address the relationship between the accumulation of RCS and the development of chronic diseases, informative methods utilizing HPLC and/or GC separations and mass spectrometry were developed, however, analyzing of multiple RCS is still a very challenging work, here we briefly summarized the pros and cons of current methods used for RCS analysis, and we further developed an LC-MS based targeted metabolomic approach for simultaneous quantifying of multiple RCS in different biomatrix, including mouse plasma and tissue samples.

Targeted profiling: Quantitative analysis of multiple reactive carbonyl species in biological samples

254th National Meeting (2017)

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