The demands for high-level energy-producing and macromolecules via metabolism alteration can be used as a promising therapeutic strategy for cancers. The main aim of this study was to investigate the anticancer activity and mechanism of isoquercitrin (ISO) induced metabolism disorder through activating AMPK signal pathways. In this study, the exposure of T24 bladder cancer cells to ISO (20-80 μM) decreased cell viability, caused ROS overproduction and Caspase-dependent apoptosis as well as metabolism dysfunction, which were regulated by the AMPK signaling pathway. Metabolic alterations identified by UHPLC-QTOF-MS were shown to evaluate metabolic pathway variation, which elucidated that lipid synthesis, protein synthesis and aerobic glycolysis were altered. The results of western blotting assay indicated that ISO had a mainly effect on metabolism disorder by AMPK activation-dependent in T24 bladder cancer cells. Our findings provided the comprehensive evidence that ISO influenced T24 bladder cancer cells metabolism and that this process was mainly involved in AMPK-activation pathway. This study could lead to an understanding of ISO suppressing cancer cells growth via other relevant biological pathways and if the affected cancer metabolism is a common mechanism by which nutritional compounds suppress cancers.

Isoquercitrin induced metabolism disorders in cancer cells by activating the AMP-activated protein kinase pathway

254th National Meeting (2017)

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