Under the current Office of Prevention, Pesticides and Toxic Substances Residue Chemistry Test Guidelines, all United States Environmental Protection Agency (US EPA) "registrants are expected to provide recovery data for parent pesticide and all metabolites of concern through" the Food and Drug Administration's (FDA) Pesticide Analytical Manual, Vol. 1 (PAM 1). The protocols described in PAM 1, Appendix II are to be followed starting with the decision tree for multiresidue methods testing. However, following the outdated decision tree and the protocols described in PAM 1 are time consuming, and in recent years, rarely lead to a primary enforcement method. A PAM multiresidue method would also have limited use when registrants are developing single analyte liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods, which quantify and confirm with a single analysis. While the stated purpose of the OPPTS 860.1360 is to use “the data generated under this guideline” so that “FDA chemists can confirm the presence or absence of many pesticides and their metabolites in commodities, and identify many unidentified analytical responses (UARs) in samples of unknown treatment history”, the challenges of analyzing for unknown pesticide residues using a multiresidue method are great. QuEChERS is an established platform for multiresidue methods in determining pesticide residues in food and feed. This presentation will discuss how a QuEChERS-based approach and use of HPLC-MS/MS can modernize the PAM multiresidue method to generate relevant data for the FDA and fulfill the US EPA requirement of OPPTS 860.1360.

QuEChERS-based approach to FDA Pesticide Analytical Manual (PAM) to fulfill the EPA requirement for Office of Prevention, Pesticides and Toxic Substances Residue Chemistry Test Guidelines OPPTS 860.1360

254th National Meeting (2017)

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