Bisphenol-A (BPA), largely used in polycarbonate plastics and polystyrene resins, is considered to be an endocrine disruptor due to its estrogenic activity. Recent extensive kinds of literature have reported the evidence on the relationship between BPA exposure and chronic human disease, including diabetes, obesity, reproductive disorders, breast cancer, birth defects, chronic respiratory and cardiovascular diseases. Therefore, a sustainably sourced, and less toxic BPA alternative is desirable for the manufacture of containers for edible products. Bisguaiacol-F (BGF) is structurally similar to BPA, with two hydroxyphenyl groups. BGF can be synthesized by reacting two derivatives of lignin and is considered as a green alternative to BPA. In this study, estrogenic activity (EA) of four isomers BGF were evaluated at six concentrations (from 10-13 to 10-8 M) by cell proliferation assay. Chemicals with EA activate the ERs and ER-dependent transcription of estrogen-responsive genes, which leads to proliferate of breast cancer cells (MCF-7 cells).The MTT assays revealed that BPA at 100pM, 1nM, and 10nM significantly promoted the in vitro proliferation of MCF-7 cells after exposure for 96 h (p&lt;0.05). The EA of BPA is dose dependent. There is no EA effect of BGF6 and BGF8 at the wide concentration range from 10-13 to 10-8 M, or of BGF1 and BGF4 at 100pM and 1 pM when compared to the no treatment group (p&lt;0.05). Even BGF1 and BGF4 at 10 nM, 1 nM, and 10 pM significantly increased the cell proliferation compared to the no treatment group, the cell proliferation was still lower than that determined at the same concentration of BPA (p&lt;0.05). All the results indicate that both BGF6 and BGF8 are the potential less toxic and sustainable alternatives to BPA.

Evaluation of estrogenic activity of the novel bisphenol-A alternative, four bisguaiacol-F compounds

254th National Meeting (2017)

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