Previous research established that the laboratory-selected 91-R strain of D. melanogaster is highly resistant to DDT (~1,500-fold more than the susceptible CS strain). Resistance was determined to be polygenic, involving factors controlling reduced penetration, increased detoxification and direct excretion. Use of UAS-RNAi transgenic lines of D. melanogaster allowed for the targeted knockdown of genes putatively involved in DDT resistance and has validated the role of several cuticular proteins (Cyp4g1 and Lcp1), cytochrome P450 monooxygenases (Cyp6g1 and Cyp12d1) and ATP binding cassette transporters (Mdr50, Mdr65, and Mrp1) involved in DDT susceptibility. The role of these seven genes directly in DDT resistance in the 91-R strain has now been validated using a nanoparticle-enhanced RNAi technique. Using this approach, the interactions of these genes are being investigated and will be summarized in this presentation.

Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches

254th National Meeting (2017)

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