Monitoring of antibiotics and other veterinary drug residues in a variety of food and environmental samples are needed for a variety of purposes, including: 1) help ensure that veterinary, food safety, and environmental regulations are being followed; 2) meet international food trade standards; 2) investigate antimicrobial resistance; 3) promote food and environmental safety; 4) asses if organic food production practices are being followed; and 5) provide survey data for risk assessment and assurances to consumers. The cost of monitoring adds to the cost of the food, and perishable food needs to be analyzed quickly, both of which places an incentive to develop and implement highly efficient and rapid methods that still meet monitoring needs. The most efficient approach in the laboratory is to analyze for as many drug analytes in as many sample types as possible at concentrations of concern. We have developed such an approach for &gt;150 diverse targeted drugs using ultrahigh performance liquid chromatography - tandem mass spectrometry (UHPLC-MS/MS), which has been implemented by the USDA Food Safety and Inspection Service, but unfortunately, aminoglycoside antibiotics are not able to be extracted at the same conditions as other drugs. Thus, two sample preparation methods are still needed, but an ion-pairing reagent can be added to a final combined extract for simultaneous analysis in a 10 min UHPLC-MS/MS method for all of the targeted drugs including aminoglycosides. This saves time and reduces the number of expensive instruments needed in the monitoring laboratory. To streamline data handling and automate both determination and identification, summation peak integration is used which yields yes/no identifications based on regulatory criteria without need for human review of chromatographic peak integrations. This method has been extensively validated in bovine liver, kidney, meat, and chicken egg products.

Streamlined analysis of >150 veterinary drugs including aminoglycosides in egg, meat, liver, and kidney samples by ultrahigh performance liquid chromatography: Tandem mass spectrometry

254th National Meeting (2017)

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