Early detection of circulating tumor cells (CTCs) from blood samples may give better opportunity for cancer patients to be cured or live longer. Lots of approaches, based on PCR, immuno-histochemistry and physical properties, are extensively studied and some of them are already adopted to clinical diagnosis. In this study, we attempted to develop fast and powerful luciferase based CTC ELISA system with EpCAM and HER2 dual targeting moiety.<br/>HER2 targeting affibodies fused with glutathione-<i>S</i>-transferease (GST) were immobilized onto glutathione (GSH) coated 96 well plate to capture HER2 overexpressing CTCs. To construct signal-amplifying secondary antibody mimic, we encapsulated luciferases into protein cage nanoparticles, encapsulin, and post-translationally ligased antibody-binding domains on their surface using SpyCatcher/SpyTag bacterial clue.<br/>Simple mix and match of functionally modulated SpyTag/SpyCatcher pairs may allow us to develop a versatile, but target-specific CTC detection system.

Developing a luciferase based circulating tumor cells detection system using functionally modulated SpyTag/SpyCatcher bacterial clue

254th National Meeting (2017)

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