FDA laboratories have been using LC-UV/fluorescence methods to monitor regulated mycotoxins in foods. Each method can only analyze for a single or single-class of mycotoxins. For suspected violations, an additional confirmation procedure using mass spectrometry (MS) is required. To gain additional information about the contamination of foods and feeds with multiple mycotoxins, this work aims to develop a single method to simultaneously monitor for multiple mycotoxins in food products.  In recent years, we have conducted a series of single- and multi-laboratory method development and validation studies to evaluate the applicability of LC-MS-based methods to the determination of mycotoxins in foods. For single-laboratory validation, we have assessed the performance of sample preparation procedures (e.g., immunoaffinity clean-up, dilute-and-shoot) in a variety of matrices with LC-MS/MS and LC-HRMS detection. For multi-laboratory studies, the FDA has validated, in collaboration with U.S. state laboratories, a stable isotope dilution LC-MS/MS method that targets aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, HT-2 toxin, T-2 toxin, and zearalenone in corn, peanut butter, and wheat flour. This newly validated method can identify and quantitate mycotoxins in different matrices using a single sample preparation procedure and LC-MS analysis. If used for routine regulatory monitoring and surveillance, this method could improve the efficiency of mycotoxin determinations. Future investigations include the evaluation of analytical standards (availability, stability, and traceability), automation of sample preparation, matrix effects, appropriate identification criteria, and harmonization of existing analytical procedures.<br/>

Fit-for-Purpose methods for mycotoxin analysis using LC-MS

254th National Meeting (2017)

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