This communication reports the grafting of recombinant diblock elastin-like polypeptides (ELPs) onto iron oxide nanoparticles (IONPs). Diblock ELPs were designed to contain a N-terminal thermosensitive block (represented in orange) undergoing a de-swelling transition at a critical temperature around 42 °C in solution fused at the C-terminal end with a hydrophilic block (represented in blue) to provide colloidal stability to the resulting γ-Fe2O3@ELP core-shell nanoparticles. Mono-core IONPs were obtained by polyol synthesis, leading to well-defined nanospheres with strong heating properties under an alternating magnetic field. The bioconjugation of diblock ELPs onto the magnetic cores was achieved in a final grafting-to step through the strong coordination bonding of a phosphonate group introduced near the N-terminus of the ELP. High temperature variations of the sample (up to 30 °C) were obtained in few minutes under magnetic hyperthermia (MH). Fast size changes of the ELP-coated IONPs were measured in situ by dynamic light-scattering when rising the temperature of the sample with an alternating magnetic field. This biocompatible system provides an interesting coupling of magnetic and thermosensitive properties for MH-induced cell death or remotely controlled drug delivery. In vitro internalization in glioma cells and cytotoxicity under MH will be presented, along with in vivo proofs of concept as MH and MRI contrast agents.

Thermosensitive diblock elastin-like polypeptides (ELPs) grafted onto magnetic iron oxide nanoparticles as dual-responsive nanomedicines

254th National Meeting (2017)

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