Interest in molecule-induced assemblies of nanomaterials has grown as a tool for the effective modulation of their properties, which can be promising sensing principles. Herein, we present a turn-on optical biosensor for the detection of MMP-2, which is based on the peptide-induced assembly of fluorescent GO nanostructure. Fluorescent GO was functionalized with a MMP-2 specific peptide substrate containing sulfhydryl group. The cross-linking of GO-Peptide nanostructures took place via the formation of disulfide bonds, which led to the sandwich assembly of GO-Peptide followed by the significant self-quenching of its inherent fluorescence. This peptide-induced GO assembly was then disassembled by proteolytic cleavage in the presence of MMP-2, leading to the efficient recovery of the self-quenched GO fluorescence. With this approach, we were able to detect MMP-2 with reaction kinetic parameters. The GO-peptide assembly was successfully applied to the sensitive detection of MMP-2 secreted by living cells.

Target-induced disassembly of GO-Peptide assemblies for the turn-on fluorescence detection of MMP-2

254th National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.