Nonlinear light scattering in the form of Second Harmonic Generation, due to its symmetry properties, has been proven effective for recording molecular transport through the membranes of living biological cells with membrane specificity and real time resolution. The transport of molecules revealed by Second Harmonic Light Scattering is confirmed by time-resolved bright-light transmission microscopy. Studies have shown that this nonlinear optical approach, unlike the patch-clamp method, can deal with biological cells with more than one membranes and charge neutral molecules as well.                                                                                                                                                         <br/>Using microscopic detection of second harmonic light scattering, we show that molecular transport through membranes of biological cells can be imaged for a single cell with time-resolution.<br/>

Imaging molecular transport through living cell membrane in real time

254th National Meeting (2017)

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