The stem, stem bark, roots and root bark of Berberis species are used in various Ayurvedic, homeopathic and ethno-medicines as raw material or as an ingredient. Selection of raw material is very important to get the desired active compounds in the prescribed range in an herbal preparation. A simple and sensitive analytical method has been developed and validated for authentication and identification of Berberis plant parts and species using HPLC fingerprinting in conjunction with chemo metric method. Diverse sets of experiments were conducted using univariate approach by selecting different extraction variables such as weight of sample, type of solvents and sample to solvent ratio, method of extraction and HPLC method. All the optimizations were done statistically using Duncan’s ranking method based on total extractive value (%) and /or berberine content (%).Identification and authentication of different parts of the plant and species was done by plotting the score plot and loading plots generated from Principle Component Analysis (PCA) using the multivariate software Unscrambler X (version 10, CAMO, USA).

HPLC fingerprinting for authentication of Berberis species

254th National Meeting (2017)

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