The emergence of bacterial resistance to antibiotics has become a topical issue globally. International guidelines require rapid detection methods to provide appropriate treatment reducing selective pressures for the proliferation of resistant strains. A common subset of antibiotic resistance involves the chemical modification of the antimicrobial into an inactive form by an enzyme expressed by the bacterium. Selected reaction monitoring (SRM) has the ability to rapidly determine these characteristic antimicrobial molecular weight shifts. Using a highly sensitive hybrid triple quadrupole/linear ion trap mass spectrometer in full MS and enhance product ion modes combined with a rugged and versatile UHPLC method, detection of acetyltransferase modification of an aminoglycoside was achieved in less than an hour.

Detection of acetyltransferase modification of aminoglycoside in bacteria using ultra-high performance liquid chromatograph-mass spectrometry

254th National Meeting (2017)

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