Soybean is an outstanding source of essential nutrients.  However, soybean is also considered a major food allergen by the Food Allergen Labeling and Consumer Protection Act (FALCPA) of 2004. Among the commercially available allergen test procedures, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a principle and important one.  In this presentation, a validated LC-MS/MS method targeting fourteen soy seed allergen proteins was successfully transferred to two mass spectrometers which have completely different geometry in the electrospray ionization source designs, and distinct sensitivities. Maintenance of assay reproducibility, including the considerations on critical reagents and equipment/apparatus, carry over controls, digestion efficiency, stock stability, extract stability, matrix stability, digestion efficiency, dilution integrity/linearity and parallelism are also summarized. Our experience demonstrated that the multiplexing LC-MS/MS method has great flexibility and adaptability, and LC-MS/MS in general is a very promising approach for allergen and plant protein quantification.

Adapting LC-MS/MS methodology for soy allergen determination using different mass spectrometers and other variables

254th National Meeting (2017)

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