Kinases play crucial roles in cell signaling, and aberrant kinase signaling has been observed in many human diseases including cancer. Kinases have also been actively pursued as molecular targets for anti-cancer therapy, where more than 100 distinct kinase inhibitors are under various stages of clinical trials for anti-cancer therapy.  Understanding cell signaling and uncovering kinase targets for anti-cancer therapy call for high-throughput methods for interrogating quantitatively the human kinome.  In this presentation, we will discuss our recent efforts toward the development of targeted quantitative proteomic approaches for assessing the human kinome in cancer cells and tissues.  In addition, the application of the kinome profiling methods for uncovering novel kinase targets of clinically used kinase inhibitors, for revealing kinases involved in the promotion and suppression of cancer metastasis, and for discovering the alterations of signaling pathways arising from exposure to toxic chemicals will be described. Together, we hope to demonstrate that our targeted quantitative proteomic methods offer sensitive, accurate, reproducible and high-throughput quantitative analysis of the human kinome.<br/>

Targeted quantitative proteomic approaches for interrogating the human kinome

254th National Meeting (2017)

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