Consumers demand besides high quality and safe foods a complete traceability to interrelate identifiable entities chronologically throughout the food chain. The availability of fast and reliable authentication methodologies is therefore urgently required and has driven forward the progress in analytical instruments technology as well as statistical data analysis. Technological improvements leading to high-resolution mass spectrometers with faster scanning capabilities at high resolving power have expanded the functionality beyond traditional data-dependent acquisition (DDA) approaches to targeted metabolomics with improved precision and sensitivity challenging the coefficients of variation typically observed in triple-quadrupole based mass spectrometers. The recent generation of tandem quadrupole time-of-flight (QqTOF) mass spectrometers led to the development and advancement of acquisition methods like high resolution multiple reaction monitoring (MRM-HR), information-dependent acquisition (IDA), sequential window acquisition of all theoretical fragment-ion spectra (SWATH), and MS<sup>All</sup>. The presentation will demonstrate and compare the application of the aforementioned data acquisition strategies for application in food authenticity studies and outline current limitations and possibilities.<br/>

Novel approaches in high-resolution UHPLC-MS based metabolomics for analysis of food authenticity

254th National Meeting (2017)

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