Nature of Residue studies (NORs) are a crucial component of pesticide development. The studies provide insight into metabolic pathways, residues at harvest in raw agriculture commodities (RACs), metabolites that require toxicological evaluation and molecules to include in analytical methods. A NOR was conducted on an experimental insecticide which resulted in a complex metabolic profile in many of the edible tissues of the laying hen. Due to the complexity of the metabolic profile, initial chromatography conditions required redevelopment. This was determined through a new method of metabolite isolation and identification by fraction collection coupled with UPLC. Isolated fractions were further identified using mass spectrometry. To ensure accurate identification of the metabolic profile from laying hens, the test material was prepared with a unique isotopic fingerprint. The increased resolution of the chromatography method and the unique isotopic fingerprint had a significant impact on the conduct of the study. This research validates the need for a unique isotopic fingerprint in the test material, particularly when a complex metabolic profile is suspected. Our research in the laying hen demonstrates the importance of high resolution separation chromatography for high quality NOR studies and its impact on the overall conclusions of a study.

Isolation and identification of a complex insecticide metabolic profile in laying hens

254th National Meeting (2017)

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