A portable optical biosensor was developed for in-field detection of Salmonella Typhimurium in poultry supply chains. Magnetic nanoparticles (100 nm) conjugated with specific antibodies were used in immunoseparation of target pathogens from complex matrices and quantum dots (emission wavelength of 620 nm) conjugated with specific aptamers were used to label target pathogens. A prototype of the biosensor system was designed and constructed as a semi-automated instrument with magnetic separation and fluorescent measurement controlled by a laptop with LabVIEW software. Different samples from a poultry supply chain, including feed, water and chickens on farm, chicken carcasses and processing water in a processing plant, and chicken products on supermarkets, were tested using the developed biosensor. The results indicated that the biosensor could detect Salmonella Typhimurium at concentrations from 102 to 106 cfu/ml within 1 h without pre-enrichment of samples. The nanomaterials-based biosensor showed great potentials for in-field rapid detection of Salmonella Typhimurium in poultry supply chains. It is being integrated with GPS, wireless and image processing to provide the food industry an innovative technology to monitor microbial contamination in a food supply chain for ensure food safety.

Nanomaterials-based biosensor system for rapid detection of Salmonella Typhimurium in poultry supply chains

254th National Meeting (2017)

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