Sepsis is an acute disease with high mortality risk. It happens more frequently than heart attack and kills more people than cancer every year. However it could be monitored by patient’s Glycosaminoglycans (GAGs) level, it has been proved GAGs level would be dramatically increased after septic shock. An ultrasensitive detection of GAGs could help in the diagnosis of septic shock in the very early stage, thus enhance the patient’s survival rate. Rolling circle amplification (RCA) is a DNA replication technique, which can generate a long single DNA strand with multiple repeat units. The detection signal of GAGs can be amplified through nucleic amplification, the limit of detection of this method has reached 100 fMol, and with a good potential to be even lower.

Ultrasensitive detection of glycosaminoglycans by rolling circle amplification

254th National Meeting (2017)

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