Thiols play a significant role in cell structure and function. Thiols have been found intracellularly, in extracellular fluids, and on cell surface. Thiol density on cell surface is easily affected by extracellular oxidant insults. Therefore, an agent that can map thiol distribution on cell surface in live cells will be a valuable tool in understanding how cells respond to oxidative stress stimuli. We would like to present the design and synthesis of thiol specific fluorogenic agents for imaging thiols on cell surface in live cells through fluorescence microscopy. These agents are designed based on a benzofurazan sulfide structure previously reported by us to be thiol specific and fluorogenic. Imaging of an analyte in live cells through fluorescence microscopy enables us to visualize the analyte in its native environment without breaking the cell and reveals information that cannot be obtained from broken cells.

Development of thiol specific fluorogenic agents for cell surface thiol imaging in live cells

254th National Meeting (2017)

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