Thiols play significant roles in various cellular functions. A change in thiol concentration has been linked with various disease states. Numerous reagents have been developed to detect thiols in biological samples. However, few are able to detect and quantify thiols in live cells, especially in mitochondria of live cells. Live cell thiol detection has the advantage of revealing information that cannot be revealed from thiol quantification of homogenized samples. BiROS was designed and synthesized as a thiol specific and mitochondria selective fluorogenic agent. BiROS effectively imaged and quantified thiols in mitochondria in live NCI-H226 cells, and effectively detected thiol change, created by a thiol-modulating agent NEM, in mitochondria at a concentration that was nontoxic to the cells. The mitochondrial thiol imaging was confirmed through a co-localization experiment with DIOC6 (3) - a mitochondria imaging agent. BiROS will be a valuable tool for the study of thiols’ function in mitochondria.

Development of bis{N,N'-rhodamine-7,7'-aminosulfonyl(benzo[c][1,2,5]oxadiazol-4-yl)}sulfane (BiROS) as a thiol specific fluorogenic agent for mitochondrial thiol imaging in live cells

254th National Meeting (2017)

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