Although larger pore volume is thermodynamically advantageous in Size Exclusion Chromatography (SEC) and is thought to be a necessity for high resolution separations by molecular size, superficially porous particles (SPPs) have kinetic advantages over fully porous particles (FPPs) for use in SEC (1). A comparison is made using FPPs and SPPs with similar particle diameters, pore size and equal flow rates using polystyrene solutes. A complete set of experimental data characterization metrics show that SPPs offer similar peak capacities to FPPs but with faster operation1 because SPPs produce more plates and more plates per unit time than FPPs.<br/><br/>This presentation will show applications of this technology including fast gold nanoparticle characterization, 2DLC with SEC in the second dimension, mAb light chain-heavy chain digests, and analysis of industrial polymers.

Size exclusion chromatography with superficially porous particles

254th National Meeting (2017)

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