Sulfated polysaccharides (SPs) were extracted from the green algae, <i>Ulva lacutca. </i>High molecular weight SPs were enzymatically hydrolysed using viscozyme then fractionated into 8 different molecular weight fractions (F1-F8) by means of diethyl amino ethyl (DEAE) anion exchange column chromatography. Crude SPs together with the hydrolysate and the 8 column effluent fractions were tested for their carbohydrate, protein and sulfate contents. In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR). Their antioxidant and antitumor activities were also determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) (MTT) assays, respectively. The fractions S1, F4, F5 and F8 showed promising antioxidant and antitumor activities. The remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate and sulfate contents, in addition to its comparable contents of rhamnose and glucuronic acid. Other factors influencing the biological activity were found to include chain length (DP), composition, type and arrangement of characteristic functional groups like sulfate, hydroxyl and amide. The biologically active SPs fractions could be potentially utilized in supplement food and integrative medicine applications.

Enzymatic hydrolysis and ion exchange fractionation of sulfated polysaccharides extracted from <i>Ulva lactuca</i> and evaluation of their antioxidant and antitumor activities

254th National Meeting (2017)

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Enzymatic hydrolysis and ion exchange fractionation of sulfated polysaccharides extracted from Ulva lactuca and evaluation of their antioxidant and antitumor activities