Recent years have shown an increase in the development and application of Raman spectroscopy for process monitoring and control in the biopharmaceutical industry. While Raman spectroscopy has been successfully used to predict cell densities and concentrations of conventional metabolites, limited work has been presented on its capability to measure product-related attributes, including titer and product quality. Here, we present results using Raman spectroscopy to obtain in-line measurements of CHO antibody titer as well as charge, size, and glycan species across a range of antibodies including complex molecular formats. In addition, we discuss the technical challenges in deriving these measurements using multivariate analysis techniques.

Technical capability of Raman spectroscopy for in-line quantification of antibody product quality

255th National Meeting (2018)

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