We report on the development of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous measurement of eight biomarkers of volatile organic compound (VOC) exposure. The metabolites, N-2-furoylglycine (N2FG), 5-hydroxymethylfuroic acid (HMFA), 2,5-furandicarboxylic acid (FDCA), 2-hydroxy-N-methylsuccinimide (HMSI), 5-hydroxy-N-methylpyrrolidone (HNMP), trans, trans-muconic acid (MUCA), S-phenylmercapturic acid (PMA), and 2-aminothiazoline-4-carboxylic acid (ATCA), can be detected in human urine. N2FG is a urinary metabolite of furfural; HMFA and FDCA are metabolites of 5-hydroxymethylfurfural; HMSI and HNMP are metabolites of N-methyl-2-pyrrolidone; MUCA and PMA are metabolites of benzene, and ATCA is a metabolite of hydrogen cyanide. Chromatographic separation was optimized using reversed-phase columns and acidic mobile phases. The overall run time for the method is five minutes per sample injection. The method has low ng/mL sensitivity, linearity over three orders of magnitude, and precision and accuracy within 15%. The method is advantageous for high-throughput analysis of selected VOC metabolites in population-based studies (e.g., National Nutrition and Health Examination Survey). Quantifying these urinary biomarkers is important to public health efforts to understand human exposure to VOCs from various sources, including tobacco products.

Quantitative measurement of furfural, 5-hydroxymethylfurfural, N-methyl-2-pyrrolidone, benzene, and hydrogen cyanide metabolites in urine using UPLC-MS/MS

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.