We report an innovative new cation exchange resin for the downstream purification of monoclonal antibodies having a unique surface chemistry that enables efficient aggregate removal in the flow-through frontal chromatography mode of operation. Operating cation exchange chromatography in the flow-through frontal chromatography mode of operation is advantageous since it allows aggregate removal to be accomplished with significantly smaller columns relative to traditional bind/elute cation exchange chromatography. We have investigated the influence of a variety of different factors (pH, conductivity, etc.) on aggregate removal using this novel cation exchange resin in a flow-through frontal chromatography mode of operation.

A new cation exchange resin designed for the removal of monoclonal antibody aggregates using flow-through frontal chromatography

255th National Meeting (2018)

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