Site specific incorporation of biorthogonal functional groups into proteins for bioconjugation techniques was successfully demonstrated with the unnatural amino acid D-ThrK. 1,2-alkanolamine-functionalizedlysines were synthesize and incorporated at an amber mutation site in green fluorescent protein (GFP) expressed in <i>Escherichia coli</i> via the coordination of pyrrolysyl-tRNA synthetase (Pyl RS) and its cognate tRNA<sup>pyl</sup>. The incorporation of this non-canonical amino acid and potential chemical transformations following it allow the synthesis of proteins with biorthogonal functional groups, making a variety of basic and biotechnological applications available.

Synthesis and incorporation of 1,2-alkanolamine-functionalized lysine as a non-canonical amino acid into GFP

255th National Meeting (2018)

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