Cancers are caused by mutations to genes that regulate cell normal functions. Most of the currently-available methods used for detection of DNA mismatch are time–consuming and/or require the use of fluorescent or radioactive labels. In this work, we developed a label-free enzyme reaction-based nanopore sensing approach to detect DNA mismatch. The method was rapid, accurate, and highly sensitive: picomolar concentrations of DNA could be detected in minutes. It worked on various DNA mutation situations, including base substitution, deletion, and insertion. In addition, simulated serum samples were successfully analyzed. Our developed nanopore DNA mutation detection strategy should find useful application in genetic diagnosis.<br/>

Nanopore based DNA mismatch detection by single-strand-specific nuclease

255th National Meeting (2018)

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