<br/>Sequence specific fluorescence sensors such as Molecular Beacon (MB) and Taqman Probe havs been widely used for real time detection of sequence specific nucleic acid, small molecules and proteins. However, limitiation of these are high background from the incomplete quenching of the fluorophore. Our recent development - split dapoxyl aptamer (SDA) probe is a highly selective label-free probe for nucleic acid detection. SDA probe consists of two DNA strands that hybridize to a target DNA or RNA sequence and form a binding site for dapoxyl, an water-soluble fluorescent solvatochromic dye with excellent photostability. When bound to the aptamer, the rotational conformational changes of Dapoxyl dye is restricted, allowing 722-fold fluorescence enhancement and a substantially low K<sub>d</sub> value of 7.6 ± 1.2 nM. SDA offers high selectivity towards single nucleotide substitutions (SNS) at ambient temperatures and can potentially be used for real time detection of NASBA product.<br/>

Real time detection of NASBA product by Split DNA aptameric sensor

255th National Meeting (2018)

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