Living systems have evolved finely tuned mechanisms to coordinate transporters, chaperones, and non-proteinaceous ligands to spatially and temporally position ions for normal physiology. In this context, freely diffusing and protein-bound anions together with anionic protein modifications link to signal transduction, regulation of intracellular pH, and even detoxification. However, a breakdown in cellular homeostasis can lead to anion dysregulation in a wide range of diseases including neuropathic pain, cancer, autism, and cystic fibrosis suggesting that anions could be signals of cellular status. Despite this biological significance, chemical biology approaches to identify the cellular stores, protein targets, and signaling roles of biologically relevant anions, are still underdeveloped, but could provide new insights for the role of anions in human health and disease. To this end, in this presentation I will describe the development and applications of turn-on fluorescent biosensors for the real-time imaging of anions in biological systems.<br/>

Optical imaging tools for elucidating the roles of anions in cellular signaling

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.