Process intensification provides many advantages for the production of therapeutic proteins. One of the strategies for process intensification is to maintain high cell densities to improve productivity. High cell density cell culture results in high demand in oxygen sparge which leads to foaming issues. This is even more prominent in intensified fed batch and intensified perfusion processes. The addition and accumulation of antifoam can cause a negative impact on cell culture performance. For better understanding, we have developed a small scale model to characterize a design space between the factors of shear, media components and additives, and antifoam addition. The goal is to minimize the effect that addition of antifoam has on cell culture growth and viability. We utilized orthogonal approaches to correlate shear stress between small scale and at scale models. We will present small scale and bioreactor data demonstrating the verification of our predicted design space.

Optimizing the addition antifoam for process intensification: A case study using small scale models

255th National Meeting (2018)

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