Nitric Oxide (NO) has previously been shown to interrupt the intrinsic coagulation pathway in vivo. Our laboratory’s prior research has shown that diamines can serve as an NO donor in vivo, and reduce or eliminate the need for classical systemic heparinizing used for an extracorporeal circuit. Therefore we have studied what the optimal storage conditions would be for polyvinyl chlorate circuits coated with a diamine and carbosil layer that releases NO at significant flux. NO flux rate is a key part in identifying the quantity of NO released by a compound to its environment and a good way to indicate effective NO availability in the blood. Previous studies have indicated that a coating of NO-donating diamine is effective at limiting coagulation in Lapine circuit models, however it is unknown how long these coated tubes can be stored for and maintain these high NO flux rates. To assess the best storage conditions for active dibutylhexanediamine (DBHD) coatings with integrated acid complexes, three conditions were assessed: 20oC, -20oC, and 37oC. These samples are assessed at multiple time points for their NO flux rate using a Sievers 280i Nitric Oxide Analyzer. Average net flux for coated tubing is 28.7 at 20oC, 34.7 at -20oC, and 34.5 at 37oC 32 days from fabrication. Average peak NO flux was 79.7 at 20oC, 105.7 at -20oC, and 69.4 at 37oC at a 32 days from fabrication. This shows that the best place to store our tubes would be in a freezer at 20o C to preserve peak NO flux, however net NO flux rate between 37oc and -20oC appear to be similar (p&gt;0.80).

Characterization of nitric oxide flux as a function of time and temperature: A durability review

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.