DNA sequences that can form alternative non-B DNA structures such as H-DNA are potential sites of chromosomal instability leading to cancer and disease. These non-B DNA-forming sequences are mutagenic and may be more susceptible to DNA damage. For example, the generation of reactive oxygen species (ROS) under oxidative stress produces DNA lesions that occur non-randomly within the genome and contribute to mutation hotspots. Here, we used a yeast artificial chromosome (YAC) containing either an H-DNA-forming sequence or the control B-form DNA to monitor the effects of DNA damage on cell survival and mutation rate in yeast. Our results suggest that DNA damage caused under ROS-generating conditions preferentially increases H-DNA-induced mutagenesis in yeast compared to B-DNA control sequences.

Effects of oxidative stress on non-B DNA structures in a yeast model system

255th National Meeting (2018)

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