Medium and feed are key factors in the success of modern high titer cell culture processes. Ensuring that all vital nutrients are available when the cells need them is a critical part of developing a commercially viable large scale production process. A key aspect of developing an upstream process is understanding the effects of scaling the unit operations and especially how the medium and feed is prepared. When the preparation methods at differing scales alter the availability of nutrients, the cell metabolism can be affected which alters cell growth, product titers, and even product quality. Recently, it has been discovered that the large-scale preparation method for a process feed has shown to have deleterious effects on its corresponding large-scale process. The most surprising discovery was that although one of the affected feed components was measurable at the expected concertation, it was not bioavailable to the cells. Details of the study and the resulting corrective actions will be discussed.

Understanding the effect of preparation methods on bioavailability of components in a cell culture feed

255th National Meeting (2018)

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