Blending pathway engineering with directed evolution, adaptive strain engineering, and synthetic biology enables a powerful approach for rapid metabolic engineering. This talk will highlight how the evolution of small parts, enzymes, pathways, and whole cells can rapidly accomplish metabolic engineering goals. This talk will describe two recent advances in speeding the design process for pathway optimization and evolution. First, this talk will discuss new approaches to multiplex gene expression within pathways and broader metabolism to identify key bottlenecks limiting flux. By utilizing a CRISPR-dCas9 system, we demonstrate a rapid method for assessing the importance of these metabolic targets in a manner that is scalable and combinatorial. Second, this talk will discuss new methods to speed and automate the processes of directed and adaptive evolution through this use of microdroplet technologies. Together, these two approaches enable rapid pathway design and optimization in yeast.

Optimization, evolution, and control of pathways and metabolism in yeasts

255th National Meeting (2018)

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