A modified diastereomer of the previously known peptide D-K6L9, SALKlLKkLlkKLLkLLAS, shows great potential against a variety of cancer types, such as glioblastoma and metastasizing breast cancer. To build a 3D model of the peptide, we have determined the solution structure of this peptide by recording 2D 1H-1H -COSY, NOESY, and TOCSY NMR spectra. A 3D model generated by CNS software utilizing NOE derived distance constraints. In addition, confocal microscopy imaging (static and live) was performed with rhodamine-B labeled peptide. Other dyes, such as Hoechst and Mitotracker Green FM, were used to help accurately find where the peptide is located. Based on these results, it is our paradigm that SALKlLKkLlkKLLkLLAS kills cancer cells by means of cell membrane destabilization and causes cell necrosis, as well as penetrating into the cells to attack mitochondria and causes cell apoptosis.

Mechanistic study of a peptide diastereomer with anti-cancer activity

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.