Research on microfluidic paper-based analytical devices (μPADs) is remarkable in recent years from the viewpoints of analytical chemistry and biochemistry. On the other hand, we can contribute to components incorporated into μPADs from the standpoint of material science. We here propose that 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-oxidized cellulose nanofibers (TOCN) can be reasonably mounted as a module of µPADs by effective use of the following features of TOCN, namely,<br/>- "oxygen barrier capability in a dry state" as "storage of unstable substances"<br/>- "exchangeability of molecules if swollen in water" as "biochemical reaction site"<br/>- "immobilization on papers" for "fixation of the sensing site"<br/>The TOCN aqueous dispersion exhibiting thixotropy can be printed with an inkjet apparatus, thus simplifying the manufacturing process of μPADs. As nanofibers derived from cellulose, which is the main component of paper, are printed on paper, incineration ash does not remain and post-treatment is hygienic. In this presentation, we report several examples of bioassay systems on paper using TOCN. Our future plans include elucidating the mechanism of stable storage of unstable molecules and extending the variety of available bio-related substances and their reactions.

Incorporation of cellulose nanofibers into μPADs: Stable storage of bio-related labile substances and function as a biochemical reaction field

255th National Meeting (2018)

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