Chromatography is commonly an integral part in downstream processing of biological human therapeutics. In the last 20 years the development of solid phases designed for purification of biological nanoplexes (e.g. viruses, virus like particles (VLPs), phages and polynucleotides) based on monoliths and membranes greatly advanced. The main advantage of these matrices are the fully convective mass transport due to the micron-sized flow channels, the absence of void volumes, and the high mass capacities.<br/>Steric exclusion chromatography (SXC) based on cellulose membranes makes full use of these advantages. In addition, no specific ligands are required to capture particles from complex mixtures. Its selectivity is strongly based on the target size. SXC is carried out by mixing the clarified cultivation broth with polyethylene glycol (PEG) and loading it onto a cellulose membrane. Based on the applied PEG molecular weight and concentration, the virus particles are captured without a direct chemical interaction by the mutual steric exclusion of PEG between the target particle and the stationary phase. Smaller impurities i.e. host cell proteins and DNA are washed out. The product is eluted by reducing the polymer (PEG) concentration and can be recovered in a variety of buffers. <br/>Here, we compare the application of classical membrane adsorbers with SXC (cellulose membranes) for the purification of oncolytic measles virus in terms of infective virus yield, contaminant depletion, productivity, and process economics.<br/>

Purification of cell culture-derived oncolytic measles virus by membrane chromatography

255th National Meeting (2018)

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