Onset of Celiac Disease (CeD) symptoms occurs due to an inflammatory response caused by the digestion of gluten products. Gliadin (and fragments thereof), which is a component of gluten and a known epitope, has a net positive charge at intestinal pH. While it has been reported in the literature that high molecular weight anionic polymers are able to bind to gliadin and reduce the overall inflammatory response, the mechanism of the interaction is not well understood. Previous studies in our laboratories established experimental conditions to synthesize poly(2-acrylamido-2-methylpropane sulfonate) (polyAMPS) and poly(3-methylpropyl-3-methylbutonate) (polyAMBA) at low degrees of polymerization (DP). These conditions were used to synthesize polyAMPS and polyAMBA at target DPs of 25, 15, and 5. PolyAMPS and polyAMBA were selected for use as polymer binders to gliadin and other food based proteins in order to test the effects of anionic strength and hydrophobic character on polymer/protein interactions at the molecular level for use as therapeutic agents in proteopathic diseases.

Synthesis of anionic acrylamide-based polymers for determination of structure/binding interactions with food-based proteins

255th National Meeting (2018)

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